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 Table of Contents  
ORIGINAL ARTICLE
Year : 2015  |  Volume : 8  |  Issue : 1  |  Page : 1-5

To study the pattern of bacterial isolates in open fractures


Department of Orthopaedics, Himalayan Institute of Medical Sciences, Dehradun, Uttarakhand, India

Date of Web Publication13-Jun-2016

Correspondence Address:
Digvijay Agarwal
Department of Orthopaedics, Himalayan Institute of Medical Sciences, Dehradun, Uttarakhand
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-7341.183953

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  Abstract 

Introduction: Open fractures are fairly common in developing countries. Causes of open fractures vary widely including road traffic accident, fall from height, gunshot, assault, machine injuries and others. Infection is a common complication of open fractures. Chronic osteomyelitis, nonunion, loss of function or even limb loss are some serious outcome of deep fracture site infections. Primary goal in management of open fractures is prevention of infection of bone and soft tissue by early debridement, irrigation of wound and administration of broad spectrum antibiotics with stabilization of fractures. Aim: The aim of the study is to elucidate pattern of microbial isolates in open fractures so as to form rationale antibiotic regimen for treating open fractures. Methods: 70 patients were taken into study of all ages, both the sexes with open fracture classified according to Gustilo Anderson classification. Primarily wound was examined and description of the wound was recorded with 1st culture swab taken at that time followed by 2nd culture swab on 1st dressing after debridement and 3rd culture swab if infection continues further. Culture and sensitivity reports were collected for studying pattern of bacterial isolate and their sensitivity. Results: Pre-debridement cultures are of no importance. Post-debridement cultures are important in formulating an antibiotic regime. Gram negative organisms are the most probable cause of infection. Aminoglycosides are the most sensitive group of drugs in both gram positive and gram negative bacteria. Cephalosporins or quinolones should be used in combination with aminoglycosides in all cases of open fracture in our vicinity. Conclusion: All institutions and hospitals should find out the most common infecting pathogen in their environment and formulate an antibiotic policy accordingly.

Keywords: Open fractures, debridement, culture, bacteria


How to cite this article:
Agarwal D, Maheshwari R, Agrawal A, Chauhan VD, Juyal A. To study the pattern of bacterial isolates in open fractures. J Orthop Traumatol Rehabil 2015;8:1-5

How to cite this URL:
Agarwal D, Maheshwari R, Agrawal A, Chauhan VD, Juyal A. To study the pattern of bacterial isolates in open fractures. J Orthop Traumatol Rehabil [serial online] 2015 [cited 2017 Oct 16];8:1-5. Available from: http://www.jotr.in/text.asp?2015/8/1/1/183953


  Introduction Top


The serious nature of open fractures has been understood since antiquity.[1] Open or compound fractures are fractures that communicate with the outside environment through a wound.[2] They are usually caused by high-energy trauma.[3] Open fractures continue to be a very common entity. Infection at a site of traumatic wounds is a common complication of open fractures.[4]

About 60-70% of contamination of the open fractures occurs at the time of injury.[5] Bacteria originate both from the skin and outside environment.[6] In some cases, the organism is not present at the time of injury, and the wound becomes infected later.[7] The dynamics of bacterial populations in soft tissue wounds and bone differ greatly over time.[8]

The primary goal in the management of open fracture is the prevention of the infection of the bone and soft tissue. To achieve this goal, the most widely accepted treatment protocols include early surgical debridement, irrigation of open wounds, administration of broad-spectrum antibiotics, and stabilization of fractures.[9]

In this study, we studied the pattern of bacterial isolates in all cases of open fractures of extremities that came to our hospital.


  Materials and Methods Top


In the present descriptive observational study, seventy consecutive patients of all ages, both the sexes, with open fracture of all the grades as per Gustilo-Anderson classification,[10] coming to Orthopaedic Outpatient Department and Emergency of Himalayan Institute of Medical Sciences were selected.

All patients with open fracture, who had taken definitive treatment before coming to our hospital or patients having diabetes mellitus were excluded from the study.

All the patients meeting the inclusion criteria were selected to study bacterial flora in open fractures and their antibiotic sensitivity after taking written informed consent from the patient and Ethics Committee of the institution as well. On arrival in the emergency, wound was examined, and the description of the wound was recorded, and then sequential swabs for aerobic culture and sensitivity were taken in three phases.

  1. At the time of admission on first inspection of the wound.
  2. After debridement on first dressing of the wound.


Culture was taken immediately on the first inspection, and the patient was taken for emergency debridement because wounds were properly scrubbed and painted with antibacterial solution before debridement followed by thorough debridement and copious lavage, cultures were not taken at that time. If the wound was primarily closed on the day of debridement; then, on the first dressing of the wound if any discharge was present, culture was taken before applying any type of antibacterial solution or cleaning the wound with saline. Dressing was done in the dressing room.

  1. Third was taken if infection continued.


All the culture and sensitivity reports were collected for the pattern of bacterial isolates and their sensitivity.

All the patients after the first debridement were given antibiotics in the form of amoxicillin, clavulanic acid, and aminoglycoside according to their body weight. Later on according to the culture and sensitivity reports, antibiotics were changed if needed. Considering the sensitivity pattern of bacteria in the past, Department of Orthopedics had the protocol of using the same antibiotics.

Descriptive and summary statistics were used for presentation of data, using Microsoft excel 2007.


  Results Top


Seventy cases of open fractures of upper and lower extremity were admitted and treated over a period of 1 year. Out of these 70 patients, 63 (90%) were male and 7 (10%) were female. Age of the patient ranged from of 3 to 75 years. A maximum number of patient 34 (48.57%) were found in the age group of 21-40 years. The most common cause of open fractures in our vicinity was found to be road traffic accident, accounting for 50 cases (71.42%). We found maximum cases 51 (72%) of open fractures in lower limb, among which tibia was the most common fractured bone with 34 (66.66%) cases. Nearly, 44 (62.85%) patients were brought to hospital after 6 h from the time of injury [Table 1]. Patients who were brought after 6 h showed maximum growth of bacterial isolates.
Table 1: Analysis of bacterial growth on the basis of time lapse between injury and presentation to hospital

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Predebridement cultures were taken in all seventy patients, and the presence of growth of organism was found in 36 patients (51.42%) [Table 2]. Out of the positive 36 patients, 24 (66.66%) were found to have Gram-positive bacterial growth. Coagulase-negative Staphylococcus aureus was the most common Gram-positive bacteria isolated. Gram-negative bacteria were found in 12 (33.33%) out of 36 cases, which showed different isolates occurring in same number.
Table 2: Overall bacterial growth pattern in different culture sample

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Postdebridement cultures showed growth in 24 (34.28%) patients with no growth in 46 patients [Table 2]. Out of the 24 patients, Gram-positive bacteria were isolated in 12 (50%) patients and Gram-negative in rest 12 (50%). Coagulase-negative S. aureus was the most common Gram-positive bacteria isolated with Acinetobacter calcoaceticus baumannii complex as the most common Gram-negative bacteria.

In our study, we had 14 patients in whom the discharge continued; the third culture was taken in all these 14 patients. Out of these which 10 (71.42%) patients showed growth of organism [Table 2]. Of these 10 patients, 9 (90%) showed growth of Gram-negative bacteria and 1 (10%) showed Gram-positive bacteria. A. calcoaceticus baumannii complex was the most common Gram-negative bacterial isolate.

On analysis of all the pre- and post-debridement cultures, we found high growth of isolates in predebridement cultures and reduced in postdebridement cultures. We also found that in our study infection was common in open Grade II and open Grade IIIB fracture [Table 3].
Table 3: Culture analysis based on Gustilo-Anderson classification

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  Discussion Top


It has been observed that most open fracture infections are caused by Gram-negative rods and Gram-positive staphylococci, and so antibiotics should cover both types of organism.[11] However, recently, Methicillin-resistant S. aureus has been found to be associated with open lower limb fractures in some series. The optimal antibiotic regimen to combat the infection rate with open fracture is not clear from the literature.[12] It is important that, in the setting of open fracture, antibiotics should not be considered as prophylactic. As infection commonly occurs in open fractures not treated with antibiotics, their administration should, better, be viewed as therapeutic.[13] Many studies have shown all open fractures should be treated with combination of a first-generation cephalosporin and an aminoglycoside.[14]

It has also been observed that a significant percentage of late infections occur with hospital-acquired organisms, suggesting that inoculation of pathogens occurs subsequent to the initial injury.[11]

The constantly changing local wound ecology and sampling variations led to the proposition of different ideas by different authors in the orthopedic literature. Based on the types of organism causing infection compared with those on early wound cultures, several authors have proposed that many infections of open fracture wounds are nosocomial.[7]

Wound contamination occurs with both Gram-positive and Gram-negative microorganism; therefore, the antimicrobial regimen should be effective against both the types of pathogen.[15]

In our study, on analysis of the predebridement, postdebridement, and third culture, positive predebridement culture showed maximum growth of Gram-positive bacteria. However, majority of these patients were found to have growth of different organism in their postdebridement culture reports. These positive postdebridement culture patients either showed no growth or the microbial isolate was totally different from the predebridement culture. A positive predebridement culture does not conclude that the patient is infected or going to have infection later on because of in our study only 30% of patients of positive predebridement culture showed growth of bacterial isolate in the postdebridement phase. Out of which only two showed the growth of the same organism. Similarly, negative predebridement cultures does not rule out the probability of infection later on, as many cases negative for growth of organism in predebridement phase showed growth of organism in postdebridement cultures. P value of the results of predebridement and postdebridement was calculated using Chi-square test and it came out to be 0.468, which was insignificant. These findings in our study led us to conclusion that predebridement cultures are of no importance. Our findings supported the observation by Faisham et al.[16] and Lee,[11] who concluded that predebridement cultures are of little predictive value.

In our study, postdebridement cultures were more representative then predebridement. Postdebridement culture in our study showed growth of both Gram-positive and Gram-negative bacteria in equal number; clearly indicating a fact that there was increase in growth of Gram-negative bacterial isolates. Another important aspect was that 80% of the patients who showed growth of the bacterial isolates in third culture or who showed signs of continued infection were found to have bacterial growth in their postdebridement culture. This finding in our study led us to the conclusion that postdebridement culture is best to formulate a proper antibiotic regimen according to the sensitivity pattern found in our vicinity for all patients with open fractures. Our finding of postdebridement culture being important coincides with study by Faisham et al.[16]

Third culture analysis showed an increase in growth of Gram-negative bacteria, namely Acinetobacter, Pseudomonas, Enterobacter, and  Escherichia More Details coli. Ninety percent bacterial isolates were Gram-negative, probably indicating nosocomial infection because growth of organism was different from postdebridement culture. This finding coincides with finding of Lee [11] and Merritt [17] who were of the opinion that infection in open fractures are of nosocomial origin as causative microorganism of infection are different to that found in initial smears. Nosocomial organisms have emerged as the main source of infection in open fractures in the developed world.[18]Pseudomonas and Enterobacter spp. are associated with hospital-acquired infection rather than initial contamination of the open fracture in the field.[19]Acinetobacter spp. is the most important nosocomial pathogen as it survives in dry environment and is multiple drug resistant.[20]Acinetobacter spp. is ubiquitous in the environment and transmitted through hands, clothing, contaminated surgical instruments, and air conditioning or ventilation devices.[21]

On analysis of predebridement, postdebridement, and subsequent culture pattern in our study, we found that open fractures of tibia showed more growth of pathogens as compared to growth seen in open fractures involving any other bone. The high susceptibility can be explained on the basis of severe comminution, contamination, and devitalization due to superficial location, subcutaneous characteristic, delay in providing early coverage, and most importantly delay in getting proper medical care at right time, which coincides with the finding of Clancey and Hansen [22] and Ikem et al.[7]

Lee [11] concluded in his study that both pre- and post-debridement cultures have essentially no value and is an unnecessary expense to the patient and hence should not be used. Our study also shows no significant correlation between pre- and post-debridement cultures, but we found postdebridement culture to be important in formulating an antibiotic regimen to be started early in emergency and subsequent cultures to be taken as long as there is any discharge from the wound site, so as to study the bacterial isolate and its sensitivity pattern to change antibiotics if necessary.

Based on the results, we would like to reemphasize that all patients with open fractures need to be assessed individually, and the basic principles of open fracture management including wound debridement, fracture stabilization, and soft tissue cover must be carried out with culture samples taken at appropriate time.


  Conclusion Top


Predebridement cultures are of no importance in treating open fractures. Postdebridement cultures are important in formulating an antibiotic policy to be started in patients of open fractures as soon as possible. Gram-negative organisms are the most probable cause of infection in cases of open fracture. Our antibiotic policy should cover both Gram-positive and Gram-negative organisms with two antibiotic drug regimen if possible. Aminoglycosides are the most sensitive group of drugs in both Gram-positive and Gram-negative bacteria. Quinolones or cephalosporins should be used in combination with aminoglycosides in all cases of open fracture in our vicinity. Absolutely, considering the results of the study, we have change the antibiotic choice and regimen in our department.

We would like to suggest that, all institutions and hospitals should find out the most common infecting pathogen in their environment and formulate an antibiotic policy accordingly.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
  References Top

1.
Anglen JO. Comparison of soap and antibiotic solutions for irrigation of lower-limb open fracture wounds. A prospective, randomized study. J Bone Joint Surg Am 2005;87:1415-22.  Back to cited text no. 1
    
2.
Hauser CJ, Adams CA Jr., Eachempati SR; Council of the Surgical Infection Society. Surgical Infection Society guideline: Prophylactic antibiotic use in open fractures: An evidence-based guideline. Surg Infect (Larchmt) 2006;7:379-405.  Back to cited text no. 2
    
3.
Zalavras CG, Marcus RE, Levin LS, Patzakis MJ. Management of open fractures and subsequent complications. J Bone Joint Surg Am 2007;89:884-95.  Back to cited text no. 3
    
4.
Lawrence RM, Hoeprich PD, Huston AC, Benson DR, Riggins RS. Quantitative microbiology of traumatic orthopedic wounds. J Clin Microbiol 1978;8:673-5.  Back to cited text no. 4
    
5.
Cat T, Hall L. Trauma: Antibiotics in open fractures. Hosp Pharm 2007;42:413-6.  Back to cited text no. 5
    
6.
Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 2001;14:244-69.  Back to cited text no. 6
    
7.
Ikem IC, Oginni LM, Bamgboye EA, Ako-Nai AK, Onipede AO. The bacteriology of open fractures in Ile-Ife, Nigeria. Niger J Med 2004;13:359-65.  Back to cited text no. 7
    
8.
Zuluaga AF, Galvis W, Jaimes F, Vesga O. Lack of microbiological concordance between bone and non-bone specimens in chronic osteomyelitis: An observational study. BMC Infect Dis 2002;2:8.  Back to cited text no. 8
    
9.
Gustilo RB. Use of antimicrobials in the management of open fractures. Arch Surg 1979;114:805-8.  Back to cited text no. 9
    
10.
Gustilo RB, Mendoza RM, Williams DN. Problems in the management of type III (severe) open fractures: A new classification of type III open fractures. J Trauma 1984;24:742-6.  Back to cited text no. 10
    
11.
Lee J. Efficacy of cultures in the management of open fractures. Clin Orthop Relat Res 1997;339:71-5.  Back to cited text no. 11
    
12.
Griffin M, Malahias M, Khan W, Hindocha S. Update on the management of open lower limb fractures. Open Orthop J 2012;6:571-7.  Back to cited text no. 12
    
13.
Zalavras CG, Patzakis MJ. Open fractures: Evaluation and management. J Am Acad Orthop Surg 2003;11:212-9.  Back to cited text no. 13
    
14.
Zalavras CG, Patzakis MJ, Holtom PD, Sherman R. Management of open fractures. Infect Dis Clin North Am 2005;19:915-29.  Back to cited text no. 14
    
15.
Sen RK, Murthy N, Gill SS, Nagi ON. Bacterial load in tissues and its predictive value for infection in open fractures. J Orthop Surg (Hong Kong) 2000;8:1-5.  Back to cited text no. 15
    
16.
Faisham WI, Nordin S, Aidura M. Bacteriological study and its role in the management of open tibial fracture. Med J Malaysia 2001;56:201-6.  Back to cited text no. 16
    
17.
Merritt K. Factors increasing the risk of infection in patients with open fractures. J Trauma 1988;28:823-7.  Back to cited text no. 17
    
18.
Okike K, Bhattacharyya T. Trends in the management of open fractures. A critical analysis. J Bone Joint Surg Am 2006;88:2739-48.  Back to cited text no. 18
    
19.
Garazzino S, Aprato A, Maiello A, Massé A, Biasibetti A, De Rosa FG, et al. Osteomyelitis caused by Enterobacter cancerogenus infection following a traumatic injury: Case report and review of the literature. J Clin Microbiol 2005;43:1459-61.  Back to cited text no. 19
    
20.
Dhawan B, Mohanty S, Das BK, Kapil A. Bacteriology of orthopaedic wound infections in an Indian tertiary care hospital. Indian J Med Res 2005;121:784-5.  Back to cited text no. 20
    
21.
Purghel F, Badea R, Ciuvica R, Anastasiu A. The use of antibiotics in traumatology and orthopaedic surgery. Clin Med 2006;1:58-65.  Back to cited text no. 21
    
22.
Clancey GJ, Hansen ST Jr. Open fractures of the tibia: A review of one hundred and two cases. J Bone Joint Surg Am 1978;60:118-22.  Back to cited text no. 22
    



 
 
    Tables

  [Table 1], [Table 2], [Table 3]



 

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